Purified Mouse Anti-MLH-1 with Control

產品名稱:Purified Mouse Anti-MLH-1 with Control

Purified Mouse Anti-MLH-1 with Control 產品介紹：

Material Number: 551092
Size: 150 μg
Reactivity: QC Testing: Human
Component: 51-1327GR
Description: Purified Mouse Anti-Human MLH-1
Size: 50 μg (3 ea)
Concentration: 0.25 mg/ml
Clone Name: G168-15
Immunogen: Human recombinant MLH
Isotype: Mouse IgG1
Target MW: 80-85 kDa
Storage Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium 
azide.
Component: 51-16526N
Description: Jurkat Cell Lysate
Size: 50 μg (1 ea)
Concentration: 1.0 mg/ml
Storage Buffer: SDS-PAGE buffer (62mM Tris pH 6.8, 2% SDS, 0.9% b-mercaptoethanol, 
0.003% bromophenol blue, 5% glycerol)

The repair of mismatched DNA is essential to maintaining the integrity of genetic information over time. In bacteria the DNA repair process is 
accomplished by the MutL, MutH, and MutS proteins. The MutS protein initially recognizes and binds to mismatched DNA. Following this, MutH, an endonuclease, and MutL form a complex with MutS and carry out an excision repair mechanism. When bacteria are deficient in one of these enzymes a mutator phenotype arises characterized by genetic instability. The important role played by DNA repair enzymes is emphasized by the fact that they are highly conserved from bacteria to yeast to mammals. In yeast the proteins are called MutS homolog 2 (MSH2), MutL homolog (MLH1), and PMS1 which is also a homolog of MutL. MSH2 is involved in the initial recognition of mismatched nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMS1 which together help facilitate the later steps in mismatch repair. Biochemical studies of the human homologs of DNA mismatch repair enzymes MLH1, PMS2, and MSH2 indicate that human MSH2 protein can bind mispaired DNA, and that human MLH1 and PMS2 can exist as a heterodimer. These and other studies support the conservation of eukaryotic DNA mismatch repair mechanisms. The G168-15 antibody recognizes human MLH1 (80-85 kDa). Full-length human recombinant MLH was expressed as a fusion protein, affinity purified, and used as immunogen.

Purified Mouse Anti-MLH-1 with Control Preparation and Storage：

Store undiluted at -20°C.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Purified Mouse Anti-MLH-1 with Control Suggested Companion Products：

Catalog Number Name Size Clone
611451 Jurkat Cell Lysate 500 μg (none)
550838 Purified Mouse Anti-Human MLH-1 1.0 ml G168-15
554073 Purified Mouse Anti-Human MLH1 0.1 mg G168-728
554002 HRP Goat Anti-Mouse Ig 1.0 ml (none)