PerCP-Cy?5.5 Rat Anti-Mouse CD45R/B220

產品名稱:PerCP-Cy?5.5 大鼠抗小鼠 CD45R/B220 抗體；PerCP-Cy?5.5 Rat Anti-Mouse CD45R/B220

中文名稱:PerCP-Cy?5.5 大鼠抗小鼠 CD45R/B220 抗體

英文名稱:PerCP-Cy?5.5 Rat Anti-Mouse CD45R/B220

PerCP-Cy?5.5 Rat Anti-Mouse CD45R/B220 產品介紹：

Material Number: 552771
Size: 0.1 mg
Concentration: 0.2 mg/ml
Clone: RA3-6B2
Immunogen: Mouse Abelson Leukemia Virus-Induced pre-B tumor cells
Isotype: Rat IgG2a, κ
Reactivity: QC Testing: Mouse
Tested in Development: Human
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide


PerCP-Cy?5.5 Rat Anti-Mouse CD45R/B220 Description：

The rat anti-mouse CD45R antibody (clone RA3-6B2) has been reported to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. It is expressed on B lymphocytes at all stages from pro-B through mature and activated B cell, but it is decreased on plasma cells and a subset of memory B cells. The levels of CD45R expression on the B-cell lineage appear to be developmentally regulated. It is also reportedly found on the abnormal T cells involved in the lymphadenopathy of lpr/lpr and gld/gld mutant mice, on lytically active subsets of lymphokine-activated killer cells (NK cells and non-MHC-restricted CTL), on apoptotic T lymphocytes of mice injected with bacterial superantigen, on a population of NK-cell precursors in the bone marrow, and on B-lymphocyte, T-lymphocyte, and macrophage progenitors in fetal liver. The CD45R antigen has been reported not to be on hematopoietic stem cells, naive T lymphocytes, or MHC-restricted CTL. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: Its intracellular (COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), plus differing levels of glycosylation. The CD45 isoforms detected in the mouse are cell type-, maturation, and activation state-specific. The CD45 isoforms play complex roles in T-cell 
and B-cell antigen receptor signal transduction. CD45R is commonly used as a pan B-cell marker; however, CD19 expression, detectable by the rat anti-mouse CD19 antibody (clone 1D3), is reported to be more restricted to the B-cell lineage. The rat anti-mouse CD45R antibody (clone RA3-6B2) has been reported to enhance isotype switching during in vitro B-cell responses and to inhibit in vivo B-cell responses. Cross-reaction of the RA3-6B2 clone with activated human T lymphocytes has also been reportedly observed.

PerCP-Cy?5.5 Rat Anti-Mouse CD45R/B220 Preparation and Storage：

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.