Purified NA/LE Rat Anti-Mouse CD8a

產品名稱:Purified NA/LE 大鼠抗小鼠 CD8a 抗體；Purified NA/LE Rat Anti-Mouse CD8a

中文名稱:Purified NA/LE 大鼠抗小鼠 CD8a 抗體

英文名稱:Purified NA/LE Rat Anti-Mouse CD8a

Purified NA/LE Rat Anti-Mouse CD8a 產品介紹：

Material Number: 553026
Alternate Name: Ly-2, Lyt-2
Size: 0.5 mg
Concentration: 1.0 mg/ml
Clone: 53-6.7
Immunogen: Mouse thymus / spleen Cells
Isotype: Rat (LOU) IgG2a, κ
Reactivity: QC Testing: Mouse
Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 
0.2μm sterile filtered. Endotoxin level is ≤0.01 EU/μg (≤0.001 ng/μg) of 
protein as determined by the LAL assay.

The 53-6.7 antibody has been reported to react with the 38 kDa α and 34 kDa α' chains of the CD8 differentiation antigen (Ly-2 or Lyt-2) of all mouse strains tested. The CD8 α and α' chains (CD8a) form heterodimers with the CD8 β chain (CD8b, Ly-3, or Lyt-3) on the surface of most thymocytes. A subpopulation of mature T lymphocytes (i.e., MHC class I-restricted T cells, including most T suppressor/cytotoxic cells) expresses almost exclusively the CD8 αβ heterodimer (the α' chain is absent). Subsets of γδ TCR-bearing T cells, intestinal intrapithelial lymphocytes, and dendritic cells express CD8a without CD8b. It has been suggested that the expression of the CD8a/CD8b heterodimer is restricted to T lymphocytes which matured in the thymus or in an extrathymic environment that had been influenced by thymus-initiated neuroendocrine signals. CD8 is an antigen coreceptor on the T-cell surface which interacts with MHC class I molecules on antigen-presenting cells or epithelial cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck (p56 [lck]). The CD8 α and α' chains arise from alternatively spliced messengers of a single CD8a gene. The longer α form associates with p56 [lck] via a CXCP motif in its cytoplasmic domain, which it shares with CD4, but not with CD8b. The truncated α' chain is unable to associate with p56 [lck], and it may function to attenuate the CD8-mediated costimulatory signal during intrathymic T-cell maturation. In vivo 
and in vitro treatment with 53-6.7 mAb has reportedly been effective at depleting CD8+ peripheral T lymphocytes. The 53-6.7 antibody has also been reported to cross-reaact with CD8 α- and α'-like polypeptides on subsets of thymic and peripheral lymphocytes in the Egyptian toad, Bufo regularis.

Purified NA/LE Rat Anti-Mouse CD8a Preparation and Storage：

Store undiluted at 4°C.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
This preparation contains no preservatives, thus it should be handled under aseptic conditions.