PE Rat Anti-Mouse CD62L

產品名稱:PE 大鼠抗小鼠 CD62L 抗體；PE Rat Anti-Mouse CD62L

中文名稱:PE 大鼠抗小鼠 CD62L 抗體

英文名稱:PE Rat Anti-Mouse CD62L

PE Rat Anti-Mouse CD62L 產品介紹：

Material Number: 553151
Alternate Name: Sell; L-selectin; LECAM-1; LAM-1; Lnhr; Ly-22; Ly-m22; Lyam-1
Size: 0.2 mg
Concentration: 0.2 mg/ml
Clone: MEL-14
Immunogen: C3H/eb mouse B lymphoma 38C-13
Isotype: Rat (F344) IgG2a, κ
Reactivity: QC Testing: Mouse
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

The MEL-14 monoclonal antibody specifically binds to CD62L (L-selectin), a 95 kDa (on neutrophils) or 74 kDa (on lymphocytes) receptor 
with lectin-like and Epidermal Growth Factor-like domains. In the mouse, L-selectin is detected on most thymocytes, with the highest levels of 
expression on an immunocompetent subset and a population of dividing progenitor cells, and on peripheral leukocytes, including subsets of B 
and T lymphocytes, neutrophils, monocytes, and eosinophils. This member of the selectin adhesion molecule family appears to be required for 
lymphocyte homing to peripheral lymph nodes and to contribute to neutrophil emigration at inflammatory sites. L-selectin is rapidly shed from 
lymphocytes and neutrophils upon cellular activation; metalloproteinases may mediate the release of CD62L ectodomains from the cell 
surface. The level of CD62L expression, along with other markers, distinguishes naive, effector, and memory T cells. L-selectin binds to 
sialytaed oligosaccharide determinants on high endothelial venules (HEV) in peripheral lymph nodes. In vitro studies have demonstrated that 
CD34, GlyCAM-1, and MAdCAM-1, all recognized by mAb MECA-79 (anti-mouse PNAd Carbohydrate Epitope, Cat. No. 553863), may be 
ligands for CD62L. MEL-14 mAb blocks in vitro binding of lymphocytes to peripheral lymph node HEV and inhibits in vivo lymphocyte 
extravasation into peripheral lymph nodes and late stages of leukocyte rolling.

PE Rat Anti-Mouse CD62L Preparation and Storage：

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.