Purified NA/LE Rat Anti-Mouse CD25

產品名稱:Purified NA/LE 大鼠抗小鼠 CD25 抗體；Purified NA/LE Rat Anti-Mouse CD25

中文名稱:Purified NA/LE 大鼠抗小鼠 CD25 抗體

英文名稱:Purified NA/LE Rat Anti-Mouse CD25

Purified NA/LE Rat Anti-Mouse CD25 產品介紹：

Material Number: 553864
Alternate Name: Interleukin-2 receptor alpha chain; IL-2RA; IL-2Rα; Il2ra; IL-2R p55
Size: 0.5 mg
Concentration: 1.0 mg/ml
Clone: PC61
Immunogen: IL-2-dependent cytolytic mouse T-cell clone B6.1
Isotype: Rat (OFA) IgG1, λ
Reactivity: QC Testing: Mouse
Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 
0.2μm sterile filtered. Endotoxin level is ≤0.01 EU/μg (≤0.001 ng/μg) of 
protein as determined by the LAL assay.


Purified NA/LE Rat Anti-Mouse CD25 Description：

The PC61 antibody reacts with CD25, the low-affinity IL-2 Receptor α chain (IL-2Rα, p55) expressed on activated T and B lymphocytes from 
all mouse strains tested. IL-2Rα by itself is not a signaling receptor. However, it can combine with IL-2 Receptor β (CD122) and γc (CD132) 
chains to c form high-affinity, signaling receptor complexes for IL-2. Resting T and B lymphocytes and resting and activated NK cells do not 
express IL-2Rα. CD25 is transiently expressed at a low level during normal B-cell development in the bone marrow on the CD45R/B220low 
TdT- sIg- Pre-B/Pre-B-II and CD45R/B220low TdT- sIgM+ sIgD- immature B stages, but not on the CD45R/B220low TdT+ sIg- 
Pro-B/Pre-B-I stage nor on CD45R/B220high TdT- sIgM+ sIgD+ mature B cells. It is expressed at a higher level during a very early stage of 
T-cell development in fetal and ***** thymus. Peripheral CD25+CD4+ lymphocytes called regulatory T (Treg) cells are involved in the 
maintenance of self-tolerance. It has also been reported that dendritic cells express CD25, recognized by mAb 7D4 (Cat. No. 553068). The 
PC61 antibody recognizes an epitope of CD25 which is distinct from the IL-2 binding site and from those recognized by mAbs 3C7 (Cat. No. 
557364) and 7D4 (Cat. No. 553068). It blocks binding of IL-2 to CD25, presumably by inducing a conformational change in CD25.

Purified NA/LE Rat Anti-Mouse CD25 Preparation and Storage：

Store undiluted at 4°C.
This preparation contains no preservatives, thus it should be handled under aseptic conditions.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.